| 產(chǎn)品編號 | bsm-62784R |
| 英文名稱 | ICOS ligand Recombinant Rabbit mAb |
| 中文名稱 | 誘導(dǎo)協(xié)同刺激分子配體CD275重組兔單抗 |
| 別 名 | B7H2; B7RP1; CD275; GL50; ICOSL; Icoslg; LICOS. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:1000-1:2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 33 kDa |
| 檢測分子量 | 55-70 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human ICOS ligand: 250-302 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells. Could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function. SWISS: O75144 Gene ID: 23308 B7協(xié)同刺激分子及其相應(yīng)受體結(jié)合所產(chǎn)生的信號在調(diào)節(jié)T細胞活化及效應(yīng)性細胞因子分泌中起重要作用，具有負性調(diào)節(jié)作用的協(xié)同刺激分子對免疫應(yīng)答的適度進行、防止自身免疫疾病的發(fā)生具有重要作用。 B7H2可協(xié)同刺激CD14 + 細胞增殖，并分泌IL-4、IL-5、IFN-β、TNF-α、GM-CSF和IL-10等細胞因子，但不能協(xié)同刺激T細胞上調(diào)IL-2的分泌。 |
| 產(chǎn)品圖片 |
Western blot analysis of HUVEC cell lysate. Using ICOS ligand (bsm-62784R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
