| 產(chǎn)品編號 | bsm-62840R |
| 英文名稱 | Phospho-STK4/3 (Thr183/Thr180) Recombinant Rabbit mAb |
| 中文名稱 | 磷酸化蛋白激酶MST重組兔單抗 |
| 別 名 | KRS1; KRS2; MST1; MST2; STK3; STK4; YSK3. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 2D13 |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:1000-1:2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 56 kDa |
| 檢測分子量 | 60 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human STK4 around the phosphorylation site of T183: KRN-pT-VIG |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. SWISS: Q13043 Gene ID: 6789 蛋白激酶MST(Mammalian Sterile20-like Kinase) 是各種組織均有表達(dá)的絲氨酸/蘇氨酸（Serine/Threonine）蛋白激酶。屬于人絲氨酸/蘇氨酸激酶的哺乳動物STE20樣激酶(MST)，MST1和MST2在caspase蛋白酶激活的細(xì)胞凋亡的上游和下游都起作用。 細(xì)胞凋亡中MST1被caspase蛋白酶切割和激活，能誘導(dǎo)細(xì)胞凋亡形態(tài)上的改變?nèi)缛旧|(zhì)凝聚。哺乳動物STE20樣激酶2 (Mst2) 與Mst1蛋白激酶很相似,有近90%的同源性。 |
| 產(chǎn)品圖片 |
Ramos (H) cells were treated with or without Staurosporine (1μM) for 1h, Hela (H) cells were treated with or without Calyculin A (50nM) for 30 min, 25 μg total protein per lane of cell lysates (see on figure) probed with Phospho-STK4/3 monoclonal antibody, unconjugated (bsm-62840R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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