產(chǎn)品編號(hào) | bsm-62846R |
英文名稱 | Integrin Alpha V Recombinant Rabbit mAb |
中文名稱 | 整合素αV(CD51)重組兔單抗 |
別 名 | CD51; Integrin alpha five; Integrin alpha-V light chain; ITGAV; Msk8; Vitronectin receptor subunit alpha; VNRA; VTNR. |
抗體來源 | Rabbit |
克隆類型 | Recombinant |
克 隆 號(hào) | 12B7 |
交叉反應(yīng) | Human,Mouse,Rat |
產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200,ICC/IF=1:50-200,IP=1:20-50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 116 kDa |
檢測(cè)分子量 | 125 |
性 狀 | Liquid |
免 疫 原 | A synthesized peptide derived from human Integrin alpha V: 31-250/1048 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產(chǎn)品介紹 |
The alpha-V (ITGAV) integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. ITGAV:ITGB3 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling. SWISS: P06756 Gene ID: 3685 |
產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with Integrin Alpha V monoclonal antibody, unconjugated (bsm-62846R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Invasive urothelial carcinoma of the bladder; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Integrin Alpha V Monoclonal Antibody, Unconjugated(bsm-62846R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Integrin Alpha V Monoclonal Antibody, Unconjugated(bsm-62846R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Integrin Alpha V Monoclonal Antibody, Unconjugated(bsm-62846R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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