| 產(chǎn)品編號 | bsm-62853R |
| 英文名稱 | Phospho-eIF4EBP1/2/3(Thr46/Thr46/Thr32) Recombinant Rabbit mAb |
| 中文名稱 | 磷酸化eIF4E結(jié)合蛋白1/2/3重組兔單抗 |
| 別 名 | 4EBP1; 4EBP2; 4EBP3; eIF4E binding protein 1; eIF4E binding protein 2; eIF4E binding protein 3; PHAS1. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | |
| 交叉反應(yīng) | Human,Mouse |
| 產(chǎn)品應(yīng)用 | WB=1:500-1:2000,IHC-P=1:50-1:200,IHC-F=1:50-1:200,IF=1:50-1:200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 11,13 kDa |
| 檢測分子量 | 13-19 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human 4E BP1 around the phosphorylation site of T46: FST-pT-PGG |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
eIF4EBP1 is a member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. SWISS: Q13541 Gene ID: 1978 4E-BP1是一種分子量為10-12kDa 的酸性蛋白，與eIF4G 競爭結(jié)合。4E-BPs 對 eIF4E 的結(jié)合是可逆的，并依賴于 4E-BP 的磷酸化狀態(tài)。非磷酸化的4E-BP1 能與 eiF4E 牢固結(jié)合，而磷酸化的4E-BP1則不能。 而一些蛋白激酶Akt、 TOR、 MAPK、S6激酶和Cdc2是已知的能通過對35、45、69位的蘇氨酸殘基或64位的絲氨酸殘基進(jìn)行磷酸化而使4E-BP1喪失對eIF4E結(jié)合能力的激酶。然而，并非所有的磷酸化事件都能 |
| 產(chǎn)品圖片 |
Western blot analysis of 293 treated with FBS+293. Using Phospho-eIF4EBP1/2/3(Thr46/Thr46/Thr32) (bsm-62853R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Pancreatic Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-eIF4EBP1/2/3(Thr46/Thr46/Thr32) Monoclonal Antibody, Unconjugated (bsm-62853R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-eIF4EBP1/2/3(Thr46/Thr46/Thr32) Monoclonal Antibody, Unconjugated (bsm-62853R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-eIF4EBP1/2/3(Thr46/Thr46/Thr32) Monoclonal Antibody, Unconjugated (bsm-62853R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-eIF4EBP1/2/3(Thr46/Thr46/Thr32) Monoclonal Antibody, Unconjugated (bsm-62853R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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