| 產(chǎn)品編號 | bsm-62886R |
| 英文名稱 | Lamin A/C Recombinant Rabbit mAb |
| 中文名稱 | 核纖層蛋白A重組兔單抗 |
| 別 名 | 70 kDa lamin; LAMA, ; LMN1; LMN C; lamin A/C; Lamin; HGPS; EMD2. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 8H20 |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:500-5000,IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200,Flow-Cyt=1ug/Test,ICC/IF=1:50-200,IP=1:20-50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 74 kDa |
| 檢測分子量 | 63,74 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human Lamin A/C: 520-584 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Lamins are intermediate filament proteins that assemble into a filamentous meshwork, and which constitute the major components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane. SWISS: P02545 Gene ID: 4000 核膜標(biāo)志物（Nuclear Envelope Marker） 核纖層蛋白(lamin) 是緊貼核內(nèi)膜的一層厚度為20～50nm的纖維蛋白層或纖維網(wǎng)絡(luò)。核纖層與細(xì)胞質(zhì)骨架、核骨架連成一個整體，一般認(rèn)為核纖層將核被膜和染色質(zhì)提供了結(jié)構(gòu)支架。有學(xué)者研究認(rèn)為：lamin蛋白與細(xì)胞凋亡及衰老有關(guān)聯(lián)，它包括：核纖層蛋白A、核纖層蛋白B、核纖層蛋白C幾個不同亞型的蛋白。 |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with Lamin A/C monoclonal antibody, unconjugated (bsm-62886R) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Lamin A/C) monoclonal Antibody, unconjugated (bsm-62886R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-FITC) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Rabbit Anti-Lamin A/C antibody (bsm-62886R): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC (bs-60295G-FITC): 1 μg/test. Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
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