| 產(chǎn)品編號(hào) | bsm-62910R |
| 英文名稱 | CD10 Recombinant Rabbit mAb |
| 中文名稱 | CD10重組兔單抗 |
| 別 名 | Atriopeptidase; CALLA; CD10; CD10 antigen; Enkephalinase; EPN; Membrane metallo endopeptidase(neutral endopeptidase, enkephalinase, CALLA, CD10); CALLA CD10; NEP; Neprilysin; Neutral endopeptidase. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號(hào) | 6E16 |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200,IP=1:20-50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 86 kDa |
| 檢測(cè)分子量 | 100 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human Neprilysin: 50-500/750 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. SWISS: P08473 Gene ID: 4311 CD10即共同型急性淋巴細(xì)胞白血病抗原,是II型跨膜糖蛋白.CD10表達(dá)于早期B淋巴細(xì)胞,某些粒性白細(xì)胞,骨髓基質(zhì)細(xì)胞,部分上皮組織及其起源的腫瘤細(xì)胞中也有表達(dá).主要應(yīng)用于某些惡性淋巴瘤和白血病的分型.CD10是一種94kD的鋅依賴性細(xì)胞膜金屬結(jié)合蛋白，參與細(xì)胞神經(jīng)肽和激素肽的后分泌過程。它廣泛地分布于腎、肝、小腸、胎盤、脈絡(luò)膜叢、腦腺、腺皮質(zhì)和白細(xì)胞，CD10是生發(fā)中心細(xì)胞及所致淋巴瘤的標(biāo)志物。 |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with CD10 monoclonal antibody, unconjugated (bsm-62910R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD10 Monoclonal Antibody, Unconjugated(bsm-62910R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD10 Monoclonal Antibody, Unconjugated(bsm-62910R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD10 Monoclonal Antibody, Unconjugated(bsm-62910R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD10 Monoclonal Antibody, Unconjugated(bsm-62910R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD10 Monoclonal Antibody, Unconjugated(bsm-62910R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Prostate Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD10 Monoclonal Antibody, Unconjugated(bsm-62910R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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