| 產(chǎn)品編號 | bsm-62920R |
| 英文名稱 | NME1/NM23A Recombinant Rabbit mAb |
| 中文名稱 | 腫瘤轉(zhuǎn)移抑制基因重組兔單抗 |
| 別 名 | NB; AWD; NBS; GAAD; NM23; NDPKA; NDPK-A; NM23-H1; NME1. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:500-1:2000,IHC-P=1:100-1:500,IHC-F=1:100-1:500,IF=1:100-1:500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 17 kDa |
| 檢測分子量 | 17 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human NDK A: 1-152/152 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Possesses nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3'-5' exonuclease activities. SWISS: P15531 Gene ID: 4830 nm23是一種轉(zhuǎn)移抑制基因，主要用于胃腸道癌、乳腺癌、肺癌的多種惡性腫瘤的研究。大多數(shù)研究結(jié)果表明，nm23陽性表達(dá)與腫瘤轉(zhuǎn)移負(fù)相關(guān)，與病人的預(yù)后呈正相關(guān)，但也有人研究報道與轉(zhuǎn)移或預(yù)后無相關(guān)性，說明其結(jié)論仍有爭議。 |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with NME1/NM23A monoclonal antibody, unconjugated (bsm-62920R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NME1/NM23A Monoclonal Antibody, Unconjugated(bsm-62920R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NME1/NM23A Monoclonal Antibody, Unconjugated(bsm-62920R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Prostate Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NME1/NM23A Monoclonal Antibody, Unconjugated(bsm-62920R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NME1/NM23A Monoclonal Antibody, Unconjugated(bsm-62920R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NME1/NM23A Monoclonal Antibody, Unconjugated(bsm-62920R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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