| 產(chǎn)品編號 | bsm-62953R |
| 英文名稱 | PKC delta/theta Recombinant Rabbit mAb |
| 中文名稱 | 蛋白激酶C-δ/θ重組兔單抗 |
| 別 名 | ALPS3; CVID9; MAY1; nPKC-delta; nPKC-theta; PKCD; PRKCD; PRKCQ; PRKCT; Protein kinase C delta type; Protein kinase C theta type; SDK1; Sphingosine-dependent protein kinase-1; Tyrosine-protein kinase PRKCD. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 15C18 |
| 交叉反應 | Human,Mouse,Rat |
| 產(chǎn)品應用 | WB=1:500-1:2000,IHC-P=1:100-1:200,IHC-F=1:100-1:200,IF=1:50-1:200,ICC/IF=1:50-1:200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 78,82 kDa |
| 檢測分子量 | 78 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human PKC delta: 100-350 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that plays contrasting roles in cell death and cell survival by functioning as a pro-apoptotic protein during DNA damage-induced apoptosis, but acting as an anti-apoptotic protein during cytokine receptor-initiated cell death, is involved in tumor suppression as well as survival of several cancers, is required for oxygen radical production by NADPH oxidase and acts as positive or negative regulator in platelet functional responses. SWISS: Q05655 Gene ID: 5580 |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with PKC delta/theta monoclonal antibody, unconjugated (bsm-62953R) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
