| 產(chǎn)品編號 | bs-1713R |
| 英文名稱 | DAPK2 Rabbit pAb |
| 中文名稱 | 凋亡相關(guān)蛋白激酶2抗體 |
| 別 名 | Death associated protein kinase 2; DAK 2; DAK2; DAP kinase related protein 1; DAPK 2; DAPK2; Death associated protein kinase 2; DRP 1; DRP1; DRP-1; MGC119312; DAP kinase 2; DAP kinase related protein 1; DAP kinase related protein 1 beta isoform; DAP-kinas |
| 研究領(lǐng)域 | 細胞生物 信號轉(zhuǎn)導 細胞凋亡 激酶和磷酸酶 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse (predicted: Rat,Dog) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 41 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human DAPK2: 271-370/370 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Protein kinase termed (DAPK2) dependant on calcium/calmodulin (Ca2+/CaM) contains an N-terminal protein kinase domain followed by a conserved CaM-binding domain with significant homologies to those of DAP kinase, a protein kinase involved in apoptosis. Overexpression of DAPK2 significantly induced the morphological changes characteristic of apoptosis. Results indicate that DAPK2 is an additional member of DAP kinase family involved in apoptotic signaling. Function: Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Regulates both type I apoptotic and type II autophagic cell deaths signal, depending on the cellular setting. The former is caspase-dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Acts as a mediator of anoikis and a suppressor of beta-catenin-dependent anchorage-independent growth of malignant epithelial cells. May play a role in granulocytic maturation. Isoform 2 can phosphorylate MYL9. It can induce membrane blebbing and autophagic cell death. Subunit: Homodimer in its autoinhibited state. Active as monomer (By similarity). Isoform 2 but not isoform 1 can interact with ATF4. Subcellular Location: Cytoplasm. Cytoplasmic vesicle, autophagosome lumen. Tissue Specificity: Isoform 2 is expressed in embryonic stem cells (at protein level). Isoform 1 is ubiquitously expressed in all tissue types examined with high levels in heart, lung and skeletal muscle. It is expressed abundantly in cells differentiated toward granulocytes and low in undifferentiated, normal and leukemic hematopoietic cells and monocytes/macrophages. Post-translational modifications: Autophosphorylation at Ser-318 inhibits its catalytic activity. Dephosphorylated at Ser-318 in response to activated Fas and TNF-alpha receptors. Similarity: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily. Contains 1 protein kinase domain. SWISS: Q9UIK4 Gene ID: 23604 Database links:
Entrez Gene: 23604 Human
Entrez Gene: 13143 Mouse
Omim: 604727 Human
SwissProt: Q9UIK4 Human
SwissProt: Q8VDF3 Mouse
Unigene: 237886 Human
Unigene: 304472 Mouse
Unigene: 335252 Mouse
Unigene: 98325 Rat
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| 產(chǎn)品圖片 |
Sample:293T Cell Lysate at 30 ug
Primary: Anti-DAPK2 (bs-1713R) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G-HRP) at 1:5000 dilution;
Predicted band size :41 kD
Observed band size : 41 kD
Sample:
Kidney(Mouse) Lysate at 30 ug
HepG2 Cell Lysate at 30 ug
Primary: Anti-DAPK2 (bs-1713R) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G-HRP) at 1:5000 dilution;
Predicted band size :41 kD
Observed band size : 41 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
