| 產(chǎn)品編號 | bs-1859R |
| 英文名稱 | NGB Rabbit pAb |
| 中文名稱 | 腦紅蛋白/神經(jīng)血紅蛋白/神經(jīng)球蛋白抗體 |
| 別 名 | neuroglobin; NGB; NGB_MOUSE. |
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Specific References (2) | bs-1859R has been referenced in 2 publications.
[IF=3.77] Hui-xian Li. et al. PACAP-derived mutant peptide MPAPO protects trigeminal ganglion cell and the retina from hypoxic injury through anti-oxidative stress, anti-apoptosis, and promoting axon regeneration. Bba-Gen Subjects. 2021 Dec;1865:130018 IF ; mouse .
[IF=1.95] Xiaohua Du. et al. Expression and distribution of neuroglobin and hypoxia-inducible factor-1α in the adult yak telencephalon. 2021 Jun 19 WB ; Yak.
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| 研究領域 | 細胞生物 免疫學 神經(jīng)生物學 信號轉(zhuǎn)導 細胞凋亡 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Rat (predicted: Human,Mouse,Rabbit,Pig,Cow) |
| 產(chǎn)品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 17 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse NGB: 51-151/151 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes an oxygen-binding protein that is distantly related to members of the globin gene family. It is highly conserved among other vertebrates. It is expressed in the central and peripheral nervous system where it may be involved in increasing oxygen availability and providing protection under hypoxic/ischemic conditions. [provided by RefSeq, Jul 2008] Function: Involved in oxygen transport in the brain. Hexacoordinate globin, displaying competitive binding of oxygen or the distal His residue to the iron atom. Not capable of penetrating cell membranes. The deoxygenated form exhibits nitrite reductase activity inhibiting cellular respiration via NO-binding to cytochrome c oxidase. Involved in neuroprotection during oxidative stress. May exert its anti-apoptotic activity by acting to reset the trigger level of mitochondrial cytochrome c release necessary to commit the cells to apoptosis. Subunit: Monomer. Homodimer and homotetramer; disulfide-linked. Subcellular Location: Perikaryon. Tissue Specificity: Predominantly expressed in brain, the strongest expression is seen in the frontal lobe, the subthalamic nucleus and the thalamus. Post-translational modifications: A redox disulfide bond regulates the heme pocket coordination and the rate of nitrite reduction to NO. Phosphorylated in vitro by ERK1, ERK2 and PKA, and in vivo during hypoxia. Phosphorylation increases nitrite reductase activity. Similarity: Belongs to the globin family. SWISS: Q9ER97 Gene ID: 64242 Database links: Entrez Gene: 58157 Human Entrez Gene: 64242 Mouse Omim: 605304 Human SwissProt: Q9NPG2 Human SwissProt: Q9ER97 Mouse Unigene: 274363 Human Unigene: 64645 Rat Neuroglobin是一種在神經(jīng)組織和神經(jīng)沖動中攜氧、轉(zhuǎn)運氧氣和供氧的特異性蛋白,并和神經(jīng)組織微循環(huán)有關,神經(jīng)球蛋白能在缺氧的環(huán)境下保護神經(jīng)元. |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (NGB) Polyclonal Antibody, Unconjugated (bs-1859R) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
