| 產(chǎn)品編號(hào) | bs-0082R |
| 英文名稱 | CASP4 Rabbit pAb |
| 中文名稱 | 半胱胺酸蛋白酶蛋白-4抗體 |
| 別 名 | Caspase-4 subunit p20; Caspase-11; CASP-11; Protease ICH-3; Apoptosis related cysteine peptidase; Apoptotic cysteine protease Mih1/TX; CASP 4; CASP-4; CASP4; CASP4_HUMAN; CASP4_MOUSE; Caspase 4 apoptosis related cysteine peptidase; Caspase-4 subunit 2; Ca |
| 研究領(lǐng)域 | 細(xì)胞生物 免疫學(xué) 干細(xì)胞 細(xì)胞凋亡 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Zebrafish (predicted: Mouse,Rat,Rabbit,Chicken) |
| 產(chǎn)品應(yīng)用 | Flow-Cyt=2ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 20/43 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse Caspase-4 subunit p20: 201-300/373 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Caspase 4 is a 377 amino acid protein (43 kDa) that belongs to the Ced3/ICE family of cystein proteases. Caspase 4 is an "upstream" caspase with a long prodomain. It undergoes a cleavage upon activation, leading to p20 and p10 subunits which have 67% homology to mature Caspase 1 over the equivalent coding sequences. Caspase 4 is capable of cleaving itself and the p30 Caspase 1 precursor, and of inducing apoptosis in transfected insect or COS cells. In high concentrations, it can also cleave the nuclear DNA enzyme PARP but the biological relevance of this cleavage is unclear. Caspase 4 mRNA is found in most tissues examined with the exception of the brain. Highest expression is found in spleen and lung, with moderate expression in heart and liver. Low expression is observed with skeletal muscle, kidney, and testis. Function: Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves caspase-1. Subunit: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a small and a large subunit. Tissue Specificity: Widely expressed, with highest levels in spleen and lung. Moderate expression in heart and liver, low expression in skeletal muscle, kidney and testis. Not found in the brain. Post-translational modifications: The two subunits are derived from the precursor sequence by an autocatalytic mechanism or by cleavage by Caspase-8. Similarity: Belongs to the peptidase C14A family. Contains 1 CARD domain. SWISS: P70343 Gene ID: 12363 Database links: Entrez Gene: 837 Human Entrez Gene: 12363 Mouse Omim: 602664 Human SwissProt: P49662 Human SwissProt: P70343 Mouse Unigene: 138378 Human Unigene: 1569 Mouse |
| 產(chǎn)品圖片 |
Blank control:U937.
Primary Antibody (green line): Rabbit Anti-Caspase 4 p20 antibody (bs-0082R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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