| 產(chǎn)品編號 | bs-7637R |
| 英文名稱 | ICAM4 Rabbit pAb |
| 中文名稱 | 細胞間粘附分子4抗體 |
| 別 名 | CD242; CD242 antigen; ICAM-4; Icam4; ICAM4_HUMAN; Intercellular adhesion molecule 4; Landsteiner Wiener blood group glycoprotein; Landsteiner-Wiener blood group glycoprotein; LW; LW blood group protein. |
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Specific References (1) | bs-7637R has been referenced in 1 publications.
[IF=3.23] Polin, Helene, et al. "Compound heterozygosity of two novel RHAG alleles leads to a considerable disruption of the Rh complex." Transfusion 56.4 (2016): 950-955. FCM ; Human.
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| 研究領域 | 心血管 細胞生物 免疫學 信號轉導 細胞凋亡 細胞粘附分子 細胞骨架 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse (predicted: Rat,Pig,Cow,Dog,Horse) |
| 產(chǎn)品應用 | WB=1:500-2000,Flow-Cyt=1ug/test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 27 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ICAM4: 85-180/271 <Extracellular> |
| 亞 型 | |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM4 is also a ligand for alpha-4/beta-1 and alpha-V integrins. Function: ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM4 is also a ligand for alpha-4/beta-1 and alpha-V integrins. Subcellular Location: Isoform Long: Cell membrane; Single-pass type I membrane protein. Isoform Short: Secreted. Tissue Specificity: Erythrocytes. Post-translational modifications: N- and O-glycosylated. Similarity: Belongs to the immunoglobulin superfamily. ICAM family. Contains 2 Ig-like C2-type (immunoglobulin-like) domains. SWISS: Q14773 Gene ID: 3386 Database links: Entrez Gene: 3386 Human Entrez Gene: 78369 Mouse Omim: 614088 Human SwissProt: Q14773 Human SwissProt: Q9ERM2 Mouse Unigene: 706750 Human Unigene: 30220 Mouse Unigene: 222410 Rat |
| 產(chǎn)品圖片 |
Sample: placenta(Mouse) Lysate at 40 ug
Primary: Anti-ICAM4 (bs-7637R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kD
Observed band size: 45 kD
Sample: Bone (Mouse) Lysate at 40 ug
Primary: Anti-ICAM4 (bs-7637R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kD
Observed band size: 45 kD
Sample: K562 Cell (Human) Lysate at 40 ug
Primary: Anti-ICAM4 (bs-7637R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kD
Observed band size: 45 kD
Blank control:K562.
Primary Antibody (green line): Rabbit Anti-ICAM4 antibody (bs-7637R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control:K562. Primary Antibody (green line): Rabbit Anti-ICAM4 antibody (bs-7637R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control: Hela.
Primary Antibody (green line): Rabbit Anti-Phospho-c-Fos (Ser32) antibody (bs-7637R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
