| 產(chǎn)品編號 | bs-7661R |
| 英文名稱 | EAF2 Rabbit pAb |
| 中文名稱 | 睪酮調節(jié)細胞凋亡誘導和腫瘤抑制基因抗體 |
| 別 名 | BM 040; BM040; EAF 2; EAF2; EAF2_HUMAN; Ehrlich S II transcriptional activator factor; ELL Associated Factor 2; ELL-associated factor 2; Festa; Testosterone regulated apoptosis inducer and tumor suppressor; Testosterone Regulated Apoptosis Inducer And Tum |
| 研究領域 | 細胞生物 細胞凋亡 細胞周期蛋白 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Rat (predicted: Human,Mouse,Rabbit,Cow,Chicken,Dog,Horse) |
| 產(chǎn)品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 29 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human EAF2: 65-160/260 |
| 亞 型 | |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Acts as a transcriptional transactivator of TCEA1 elongation activity (By similarity). Acts as a transcriptional transactivator of ELL and ELL2 elongation activities. Potent inducer of apoptosis in prostatic and non-prostatic cell lines. Inhibits prostate tumor growth in vivo. Function: Acts as a transcriptional transactivator of TCEA1 elongation activity (By similarity). Acts as a transcriptional transactivator of ELL and ELL2 elongation activities. Potent inducer of apoptosis in prostatic and non-prostatic cell lines. Inhibits prostate tumor growth in vivo. Subunit: Isoform 1 and isoform 2 interact with TCEA1. Interacts with ELL and ELL2. Subcellular Location: Nucleus speckle. Tissue Specificity: Expressed in heart, brain, placenta, lung, skeletal muscle, kidney, pancreas, spleen, prostate, testis, small intestine, colon, adrenal, bone marrow, lymph node, spinal gland, stomach, thyroid, trachea, thymus, liver and leukocytes. Similarity: Belongs to the EAF family. SWISS: Q96CJ1 Gene ID: 55840 Database links: Entrez Gene: 55840 Human Entrez Gene: 106389 Mouse Omim: 607659 Human SwissProt: Q96CJ1 Human SwissProt: Q91ZD6 Mouse Unigene: 477325 Human Unigene: 432078 Mouse Unigene: 20681 Rat |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EAF2) Polyclonal Antibody, Unconjugated (bs-7661R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
