產(chǎn)品編號(hào) | bs-7979R |
英文名稱 | BTBD7 Rabbit pAb |
中文名稱 | BTB/POZ結(jié)構(gòu)域蛋白7抗體 |
別 名 | BTB/POZ domain-containing protein 7; BTBD7; BTBD7_HUMAN; FUP1; KIAA1525; 8030448M07; E130118E17Rik; mKIAA1525; 5730507E09Rik. |
研究領(lǐng)域 | 腫瘤 細(xì)胞生物 轉(zhuǎn)錄調(diào)節(jié)因子 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
克 隆 號(hào) | |
交叉反應(yīng) | Human,Rat (predicted: Mouse,Pig,Sheep,Cow,Dog,Horse) |
產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=0.2μg/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 126 kDa |
檢測(cè)分子量 | |
細(xì)胞定位 | 細(xì)胞核 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human BTBD7: 41-130/1132 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產(chǎn)品介紹 | |
產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Rat small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BTBD7) Polyclonal Antibody, Unconjugated (bs-7979R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Blank control (blue line): Hela (fixed with 80% methanol (5 min at -20℃) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature).
Primary Antibody (green line): Rabbit Anti- BTBD7 antibody (bs-7979R),Dilution: 0.2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC,dilution: 1μg /test.
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