| 產(chǎn)品編號(hào) | bs-13638R |
| 英文名稱 | AP1M2 Rabbit pAb |
| 中文名稱 | AP-1μ2抗體 |
| 別 名 | Adaptor protein complex AP-1 mu-2 subunit; Adaptor protein complex AP1 mu2 subunit; Adaptor related protein complex 1 mu2 subunit; Adaptor-related protein complex 1 mu-2 subunit; AP mu chain family member mu1B; AP-1 complex subunit mu-2; AP-mu chain family member mu1B; AP1 complex subunit mu2; AP1M2; AP1M2_HUMAN; AP47 2; Clathrin assembly protein complex 1 medium chain 2; Golgi adaptor HA1/AP1 adaptin mu-2 subunit; Golgi adaptor HA1/AP1 adaptin mu2 subunit; HA1 47 kDa subunit 2; HSMU1B; MU 1B; Mu adaptin 2; Mu-adaptin 2; Mu1B adaptin; Mu1B-adaptin. |
| 研究領(lǐng)域 | 細(xì)胞生物 信號(hào)轉(zhuǎn)導(dǎo) 轉(zhuǎn)錄調(diào)節(jié)因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號(hào) | |
| 交叉反應(yīng) | Human (predicted: Mouse,Pig,Sheep,Cow,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 48 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human AP1M2: 1-100/423 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the trans-Golgi network (TGN) and endosomes. The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. Subcellular Location: Golgi apparatus. Cytoplasmic vesicle > clathrin-coated vesicle membrane. Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex. Post-translational modifications: Phosphorylation of membrane-bound AP1M1/AP1M2 increases its affinity for sorting signals. Similarity: Belongs to the adaptor complexes medium subunit family. Contains 1 MHD (mu homology) domain. SWISS: Q9Y6Q5 Gene ID: 10053 Database links: Entrez Gene: 10053 Human Entrez Gene: 11768 Mouse Omim: 607309 Human SwissProt: Q9Y6Q5 Human |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Human HepG2 cell lysates
Lane 2: Human MCF-7 cell lysates
Lane 3: Human A431 cell lysates
Primary: Anti-AP1M2 (bs-13638R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kDa
Observed band size: 46 kDa
Tissue/cell: human liver carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-AP1M2 Polyclonal Antibody, Unconjugated(bs-13638R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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