| 產(chǎn)品編號 | bs-17353R |
| 英文名稱 | Homez Rabbit pAb |
| 中文名稱 | 同源盒亮氨酸拉鏈蛋白HOMEZ抗體 |
| 別 名 | Homeobox and leucine zipper protein Homez; Homeodomain leucine zipper containing factor; KIAA1443. |
| 研究領域 | 細胞生物 結(jié)合蛋白 鋅指蛋白 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse (predicted: Human) |
| 產(chǎn)品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 60 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Homez: 131-230/719 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Homeodomain-containing proteins function as transcription factors that typically switch on cascades of other genes. Usually homeodomain proteins act in the promoter region of their target genes as complexes with other transcription factors, leading to much higher target specificity than a single homeodomain protein. HOMEZ (Homeobox and leucine zipper protein) is a 525 amino acid nuclear protein that contains 3 atypical homeodomains, 2 leucine zipper-like motifs, proline and serine-rich motifs and an acidic domain. Within homeodomain 2, it contains a putative nuclear localization signal. HOMEZ shares significant sequence similarity with mouse ZHX1 and sequences that are homologous to HOMEZ are restricted to vertebrates. Likely functioning as a transcription regulator, HOMEZ is ubiquitously expressed with highest levels found in in ovary, testis, kidney, fetal lung and kidney. Function: Homez and members of ZHX family of zinc finger homeodomain factors are delineated as a subset within the superfamily of homeobox-containing proteins. Subcellular Location: Nuclear SWISS: Q8IX15 Gene ID: 57594 Database links: Entrez Gene: 57594 Human Entrez Gene: 239099 Mouse Omim: 608119 Human SwissProt: Q8IX15 Human SwissProt: Q504Z8 Mouse SwissProt: Q80W88 Mouse Unigene: 52743 Rat |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Homez) Polyclonal Antibody, Unconjugated (bs-17353R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
