| 產(chǎn)品編號 | bs-18514R |
| 英文名稱 | ZNF622 Rabbit pAb |
| 中文名稱 | 鋅指蛋白622抗體 |
| 別 名 | MGC108936; Zfp622; Zinc finger protein 622; Zinc finger-like protein 9; ZN622_HUMAN; ZNF622; ZPR9. |
| 研究領域 | 轉(zhuǎn)錄調(diào)節(jié)因子 鋅指蛋白 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse,Rat (predicted: Human,Rabbit,Sheep,Cow,Horse) |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 54 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ZNF622: 1-100/477 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
ZNF622 (Zinc Finger Protein 622) is a Protein Coding gene. Diseases associated with ZNF622 include Autosomal Dominant Non-Syndromic Intellectual Disability 44 and Autoinflammation, Panniculitis, And Dermatosis Syndrome. Gene Ontology (GO) annotations related to this gene include nucleic acid binding. Function: May behave as an activator of the bound transcription factor, MYBL2, and be involved in embryonic development. Subcellular Location: Cytoplasm. Nucleus. Tissue Specificity: Expressed in lung, kidney, spleen, liver and brain with lowest expression in kidney. Post-translational modifications: Phosphorylated by MELK. The phosphorylation may redirect the protein to the nucleus. Similarity: Contains 2 U1-type zinc fingers. SWISS: Q969S3 Gene ID: 90441 Database links: Entrez Gene: 90441 Human Omim: 608694 Human SwissProt: Q969S3 Human Unigene: 60300 Human |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with ZNF622 polyclonal antibody, unconjugated (bs-18514R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ZNF622) Polyclonal Antibody, Unconjugated (bs-18514R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
