| 產(chǎn)品編號(hào) | bs-19334R |
| 英文名稱 | NPL4 Rabbit pAb |
| 中文名稱 | 核定位蛋白4抗體 |
| 別 名 | KIAA1499; NPL4; NPL4, S. cerevisiae, homolog of; NPL4_HUMAN; nploc4; nuclear protein localization 4 homolog(S. cerevisiae); Nuclear protein localization 4 homolog; Nuclear protein localization protein 4 homolog; Nuclear protein localization protein 4, S. cerevisiae, homolog of; Protein NPL4. |
| 研究領(lǐng)域 | 細(xì)胞生物 信號(hào)轉(zhuǎn)導(dǎo) 轉(zhuǎn)錄調(diào)節(jié)因子 |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Polyclonal |
| 克 隆 號(hào) | |
| 交叉反應(yīng) | Mouse,Rat (predicted: Human,Rabbit,Pig,Sheep,Cow,Chicken,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 68 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NPL4: 271-370/608 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The NPL4 gene encodes the 608-amino acid, endoplasmic reticulum and nuclear membrane protein Npl4 that forms a complex with p97/Cdc48p and Ufd1p. This complex plays a role in IP3 receptor processing by recognizing ubiquitinated IP3 receptors in the endoplasmic reticulum and delivering them to the proteasome for degradation. The Npl4 protein contains 8 potential N-myristoylation sites, 5 potential N-glycosylation sites, several phosphorylation sites, and a C-terminal zinc finger motif. This protein is 96% homologous to the rat Npl4 protein, 44% homologous to the Caenorhabditis elegans Npl4 protein, and 34% homologous to the Saccharomyces cerevisiae Npl4 protein. Mutations in the NPL4 gene cause defects in nuclear envelope morphology, nuclear protein import, and nuclear poly(A) RNA export. Function: The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. Subcellular Location: Cytoplasm; cytosol. Endoplasmic reticulum. Nucleus. Associated with the endoplasmic reticulum and nuclear. Tissue Specificity: Expressed at highest levels in brain, heart, skeletal muscle, kidney and fetal liver. Similarity: Belongs to the NPL4 family. Contains 1 RanBP2-type zinc finger. SWISS: Q8TAT6 Gene ID: 55666 Database links: Entrez Gene: 55666 Human Entrez Gene: 217365 Mouse Omim: 606590 Human SwissProt: Q8TAT6 Human SwissProt: P60670 Mouse Unigene: 464333 Human Unigene: 309520 Mouse Unigene: 144645 Rat Unigene: 217306 Rat |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NPL4) Polyclonal Antibody, Unconjugated (bs-19334R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NPL4) Polyclonal Antibody, Unconjugated (bs-19334R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
|
