| 產(chǎn)品編號(hào) | bs-19351R |
| 英文名稱(chēng) | Neural retinal specific leucine zipper/NRL Rabbit pAb |
| 中文名稱(chēng) | 神經(jīng)視網(wǎng)膜特定亮氨酸拉鏈蛋白抗體 |
| 別 名 | D14S46E; Neural retina-specific leucine zipper protein; Neural retinal specific leucine zipper; NRL; NRL MAF; NRL_HUMAN; RP27. |
| 研究領(lǐng)域 | 細(xì)胞生物 神經(jīng)生物學(xué) 轉(zhuǎn)錄調(diào)節(jié)因子 表觀(guān)遺傳學(xué) |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Polyclonal |
| 克 隆 號(hào) | |
| 交叉反應(yīng) | Human,Rat (predicted: Mouse,Rabbit,Pig,Sheep,Cow,Dog) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 26 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Neural retinal specific leucine zipper/NRL: 151-237/237 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a basic motif-leucine zipper transcription factor of the Maf subfamily. The encoded protein is conserved among vertebrates and is a critical intrinsic regulator of photoceptor development and function. Mutations in this gene have been associated with retinitis pigmentosa and retinal degenerative diseases. [provided by RefSeq, Jul 2008] Function: Transcription factor which regulates the expression of several rod-specific genes, in cluding RHO and PDE6B. Subcellular Location: Nucleus. Tissue Specificity: Neural retina. DISEASE: Defects in NRL are the cause of retinitis pigmentosa type 27 (RP27) [MIM:162080]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP27 inheritance is autosomal dominant. Similarity: Belongs to the bZIP family. Contains 1 bZIP domain. SWISS: P54845 Gene ID: 4901 Database links: Entrez Gene: 4901 Human Entrez Gene: 18185 Mouse Omim: 162080 Human SwissProt: P54845 Human SwissProt: P54846 Mouse Unigene: 652297 Human Unigene: 20422 Mouse |
| 產(chǎn)品圖片 |
Sample: Raji (human)cell Lysate at 40 ug
Primary: Anti-NRL(bs-19351R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 26 kD
Observed band size: 30 kD
Tissue/cell: Rat eye tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;
Incubation: Anti-NRL Polyclonal Antibody, Unconjugated(bs-19351R) 1:500, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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