| 產(chǎn)品編號(hào) | bs-19506R |
| 英文名稱 | NUDCD1 Rabbit pAb |
| 中文名稱 | 腫瘤抗原CML66抗體 |
| 別 名 | 4921532K09Rik; AA407246; AW260430; AW556235; Chronic myelogenous leukemia tumor antigen 66; CML66; CML66 L; FLJ14991; NudC domain containing 1; NudC domain-containing protein 1; NUDC1_HUMAN; nudcd1; OVA66; RGD1310624; Tumor antigen CML66. |
| 研究領(lǐng)域 | 腫瘤 轉(zhuǎn)錄調(diào)節(jié)因子 |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號(hào) | |
| 交叉反應(yīng) | Mouse (predicted: Human,Rat,Rabbit,Sheep,Cow,Zebrafish,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 67 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NUDCD1: 431-530/583 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The NUDCD1 gene encodes the 583 amino acid CML66 protein which demonstrates expression in a variety of solid tumor cell lines and leukemias. Expression of CML66 in normal tissues is restricted to testis and heart. The CML66 antibody is found in sera of 18-38% of patients with lung cancer, melanoma or prostate cancer. These findings indicate that CML66 may be immunogenic in a wide variety of malignancies and may be a target for antigen-specific immunotherapy. A short isoform of CML66, designated CML66-S, is a product of alternative splicing. CML66-S has the same C-terminus as full-lenghth CML66 (also designated CML66-L), but has a unique N-terminus. CML66-S shows predominant expression in testis, and is also expressed in very low levels in tumor cells, while CML66 is expressed in tumor cells and testis. Function: Isoform 1 is specifically expressed in leukemias and a variety of solid tumor cell lines and is also detected in testis and heart. Isoform 2 is predominantly expressed in testis and weakly expressed in tumor cells. Similarity: Contains 1 CS domain. SWISS: Q96RS6 Gene ID: 84955 Database links: Entrez Gene: 84955 Human Entrez Gene: 67429 Mouse Omim: 606109 Human SwissProt: Q96RS6 Human SwissProt: Q6PIP5 Mouse Unigene: 380291 Human Unigene: 292021 Mouse |
| 產(chǎn)品圖片 |
Sample:
Thymus (Mouse) Lysate at 40 ug
Primary: Anti-NUDCD1 (bs-19506R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 67 kD
Observed band size: 63 kD
Paraformaldehyde-fixed, paraffin embedded (mouse testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NUDCD1) Polyclonal Antibody, Unconjugated (bs-19506R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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