| 產(chǎn)品編號 | bs-21173R |
| 英文名稱 | SH3GLB2 Rabbit pAb |
| 中文名稱 | 胞漿蛋白SH3GLB2抗體 |
| 別 名 | Endophilin B2; Endophilin-B2; OTTHUMP00000022320; PP6569; PP9455; Retinoid receptor induced gene 1; RRIG1; SH3 containing protein SH3GLB2; SH3 domain GRB2 like endophilin B2; SH3 domain-containing GRB2-like protein B2; SH3GLB2; SHLB2_HUMAN. |
| 研究領域 | 腫瘤 細胞生物 轉錄調(diào)節(jié)因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Mouse,Rat (predicted: Human,Rabbit,Pig,Sheep,Cow,Chicken,Dog,Horse) |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 44 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SH3GLB2: 1-100/395 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
SH3GLB2 (SH3 Domain Containing GRB2 Like, Endophilin B2) is a Protein Coding gene. Among its related pathways are Endocytosis. An important paralog of this gene is SH3GLB1. Subunit: Homodimer, and heterodimer with SH3GLB1. Subcellular Location: Cytoplasm. Tissue Specificity: Detected in skeletal muscle, adipocyte, brain, lung, colon and mammary gland. Similarity: Belongs to the endophilin family. Contains 1 BAR domain. Contains 1 SH3 domain. SWISS: Q9NR46 Gene ID: 56904 Database links: Entrez Gene: 56904 Human Entrez Gene: 227700 Mouse Omim: 609288 Human SwissProt: Q9NR46 Human SwissProt: Q8R3V5 Mouse Unigene: 460238 Human Unigene: 33343 Mouse Unigene: 26881 Rat |
| 產(chǎn)品圖片 |
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-SH3GLB2 (bs-21173R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44 kD
Observed band size: 44 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SH3GLB2) Polyclonal Antibody, Unconjugated (bs-21173R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
