| 產(chǎn)品編號 | bs-23014R |
| 英文名稱 | Caspase-12 Rabbit pAb |
| 中文名稱 | 半胱胺酸蛋白酶蛋白-12抗體 |
| 別 名 | Caspase-12; UNQ9415; Apoptosis related cysteine protease; CASP 12; casp12; CASP12P1; Caspase 12 pseudogene 1; CASP-12; casp12; caspase 12(gene/pseudogene); Caspase12; CASPC_HUMAN; OTTHUMP00000207026; OTTHUMP00000207028; OTTHUMP00000207031; OTTHUMP00000207 |
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Specific References (2) | bs-23014R has been referenced in 2 publications.
[IF=6.684] Haiying Liu. et al. Tunicamycin Induces Hepatic Stellate Cell Apoptosis Through Calpain-2/Ca2 +-Dependent Endoplasmic Reticulum Stress Pathway. Front Cell Dev Biol. 2021; 9: 684857 IHC ; rat.
[IF=2.586] Yinghua Chen. et al. FATP2 regulates non-small cell lung cancer by mediating lipid metabolism through ACSL1. TISSUE CELL. 2023 Jun;82:102105 WB ; Human.
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| 研究領域 | 細胞生物 細胞凋亡 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 38 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Caspase-12: 21-100/341 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Caspases are cysteine proteases that cleave C-terminal aspartic acid residues on their substrate molecules. This gene is most highly related to members of the ICE subfamily of caspases that process inflammatory cytokines. In rodents, the homolog of this gene mediates apoptosis in response to endoplasmic reticulum stress. However, in humans this gene contains a polymorphism for the presence or absence of a premature stop codon. The majority of human individuals have the premature stop codon and produce a truncated non-functional protein. The read-through codon occurs primarily in individuals of African descent and carriers have endotoxin hypo-responsiveness and an increased susceptibility to severe sepsis. Several alternatively spliced transcript variants have been noted for this gene. [provided by RefSeq, Feb 2011] Function: Has no protease activity. May reduce cytokine release in response to bacterial lipopolysaccharide during infections. Reduces activation of NF-kappa-B in response to TNF. Tissue Specificity: Detected in heart, kidney, liver, lung, pancreas, small intestine, spleen, stomach, thymus and testis. Similarity : Belongs to the peptidase C14A family. Similarity: Belongs to the peptidase C14A family. Contains 1 CARD domain. SWISS: Q6UXS9 Gene ID: 100506742 Database links: Entrez Gene: 100506742 Human SwissProt: Q6UXS9 Human Unigene: 476989 Human ASPASE-12是caspase家族成員，是存在于內質網(wǎng)上的促凋亡因子，內質網(wǎng)應激早期可通過GRP（glucose-regulated protein，GRP）的表達增多來保護細胞；CASPASE-12是內質網(wǎng)細胞關鍵介導因子，caspase-12還是caspase-1的抑制劑。 |
| 產(chǎn)品圖片 |
Sample: Lane 1: Stomach (Mouse) Lysate at 40 ug Lane 2: Liver (Mouse) Lysate at 40 ug Primary: Anti-Caspase-12 (bs-23014R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 45/52 kD Observed band size: 46 kD
Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Caspase-12) Polyclonal Antibody, Unconjugated (bs-23014R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
