| 產(chǎn)品編號 | bs-21604R |
| 英文名稱 | Nm23-H2/NME2 Rabbit pAb |
| 中文名稱 | 腫瘤抑制基因NME2抗體 |
| 別 名 | nucleoside dIPhosphatase kinase B; C myc purine binding transcription factor PUF; NDK B; NDKB; NDP kinase B; NDPKB; NM23 H2; NM23B; Nucleoside diphosphate kinase B; nm23-M2; NME2; Nucleoside diphosphate kinase alpha, p18-12d; puf; MGC107562; MGC107563; MGC111212; non-metastatic cells 2, protein(NM23B)expressed in; NDKB_HUMAN; C-myc purine-binding transcription factor PUF; Histidine protein kinase NDKB; nm23-H2. |
| 研究領(lǐng)域 | 腫瘤 轉(zhuǎn)錄調(diào)節(jié)因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應(yīng) | Human,Mouse (predicted: Rat,Rabbit,Pig,Sheep,Cow,Chicken) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 17 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Nm23-H2/NME2 : 1-100/152 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
NM23 is a heterodimeric protein functioning as a nucleoside diphosphate (NDP) kinase. NME1 and NME2 comprise the 152 amino acid A and B polypeptide chains of the NM23 enzyme, respectively. NME2 is identical to the beta subunit of human erythrocyte NDP kinase. NDP kinases are involved in the synthesis of nucleoside triphosphates, and NM23 may act in the regulation of signal transduction by complexing with G proteins, causing activation/inactivation of developmental pathways. NEM2 has been identified as a putative tumor suppressor. High expression of mouse NME2 is detected in heart, liver, and kidney, with moderate expression in skeletal muscle, and negligible expression in other mouse tissues examined. Function: Major role in the synthesis of nucleoside triphosphates other than ATP. Negatively regulates Rho activity by interacting with AKAP13/LBC. Acts as a transcriptional activator of the MYC gene; binds DNA non-specifically (PubMed:8392752). Exhibits histidine protein kinase activity. Subunit: Hexamer of two different chains: A and B (A6, A5B, A4B2, A3B3, A2B4, AB5, B6). Interacts with CAPN8. Interacts with AKAP13. Subcellular Location: Cytoplasm. Nucleus. Note=Isoform 2 is mainly cytoplasmic and isoform 1 and isoform 2 are excluded from the nucleolus. Tissue Specificity: Ubiquitously expressed. Similarity: Belongs to the NDK family. SWISS: P22392 Gene ID: 654364 Database links: Entrez Gene: 395916 Chicken Entrez Gene: 4831 Human Entrez Gene: 654364 Human Entrez Gene: 18103 Mouse Omim: 156491 Human SwissProt: P22392 Human SwissProt: Q01768 Mouse Unigene: 463456 Human Unigene: 1260 Mouse Unigene: 927 Rat Nm23-H2在高轉(zhuǎn)移性的腫瘤中呈現(xiàn)低表達，提示NM23-H2具有轉(zhuǎn)移抑制的功能。其抑制腫瘤轉(zhuǎn)移機制為增強微絲、微管的聚合，影響細胞的移動，從而抑制腫瘤的侵襲和轉(zhuǎn)移。 |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Nm23-H2,NME2) Polyclonal Antibody, Unconjugated (bs-21604R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Nm23-H2,NME2) Polyclonal Antibody, Unconjugated (bs-21604R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Nm23-H2,NME2) Polyclonal Antibody, Unconjugated (bs-21604R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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