| 產(chǎn)品編號 | bsm-60551M |
| 英文名稱 | FIS1 Recombinant Mouse mAb |
| 中文名稱 | 線粒體裂變1蛋白重組鼠單抗 |
| 別 名 | CGI 135; CGI135; FIS 1; FIS1; Fis1 homolog; FIS1, S. cerevisiae, homolog of; FIS1_HUMAN; Fission 1(mitochondrial outer membrane) homolog(S. cerevisiae); Fission 1(mitochondrial outer membrane) homolog(yeast); Fission 1(mitochondrial outer membrane) homolo |
| 研究領(lǐng)域 | 細(xì)胞生物 細(xì)胞凋亡 |
| 抗體來源 | Mouse |
| 克隆類型 | Recombinant |
| 交叉反應(yīng) | Human (predicted: Mouse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-1000,IHC-P=1:100-500,IHC-F=1:400-800,IF=1:20-100,ICC/IF=1:20-100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 17 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human FIS1 |
| 亞 型 | IgG1/Kappa |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The balance between fission and fusion regulates the morphology of mitochondria. TTC11 is a component of a mitochondrial complex that promotes mitochondrial fission (James et al., 2003 [PubMed 12783892]).[supplied by OMIM, Mar 2008] SWISS: Q9Y3D6 Gene ID: 51024 |
| 產(chǎn)品圖片 |
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: room temperature 2h
Secondary Ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: 1: MCF-7, 2: T47D, 3: MDA-MB-231
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 15 kDa
Observed MW: 15 kDa
Cell line: HepG2
Fixative: 4% Paraformaldehydel
Permeabilization: 0.1% TritonX-100
Primary Ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary Ab: Goat Anti-Mouse IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for bsm-60551M
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (FIS1) monoclonal Antibody, Unconjugated (bsm-60551M) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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