產(chǎn)品編號 | bsm-52660R |
英文名稱 | CCNA2 Recombinant Rabbit mAb |
中文名稱 | 周期素A2重組兔單抗 |
別 名 | CCNA2_HUMAN; Cyclin-A2; Cyclin-A; CCN1; CCN1; CCNA; cyclin A2; |
研究領(lǐng)域 | 腫瘤 心血管 細胞生物 細胞周期蛋白 |
抗體來源 | Rabbit |
克隆類型 | Recombinant |
克 隆 號 | 2C2 |
交叉反應(yīng) | Human (predicted: Mouse,Rat) |
產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:400-800,IF=1:100-500,ICC/IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 49 kDa |
檢測分子量 | |
細胞定位 | 細胞漿 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | Recombinant mouse Cyclin A2 protein: 1-240/422 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.02% Proclin300. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產(chǎn)品介紹 |
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members function as regulators of the cell cycle. This protein binds and activates cyclin-dependent kinase 2 and thus promotes transition through G1/S and G2/M. [provided by RefSeq, Aug 2016] SWISS: P51943 Gene ID: 12428 |
產(chǎn)品圖片 |
Sample:
Lane 1: Human HeLa cell lysates
Lane 2: Human Jurkat cell lysates
Lane 3: Human MOLT4 cell lysates
Lane 4: Human HL-60 cell lysates
Primary: Anti-CCNA2 (bsm-52660R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kDa
Observed band size: 49 kDa
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1、抗體溶解方法 | |
2、抗體修復(fù)方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關(guān)于肽鏈的設(shè)計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |