| 產(chǎn)品編號 | IHC0121 |
| 英文名稱 | GAPDH Ready-To-Use IHC Kit |
| 中文名稱 | 3-磷酸甘油醛脫氫酶即用型免疫組化試劑盒 |
| 性 狀 | Liquid |
| 種 屬 | Human，Mouse，Rat |
| 應(yīng)用范圍 | Immunohistochemistry |
| 適用樣品基質(zhì) | FFPE tissue |
| 描述 | IHC0121 is a ready-to-use IHC kit for staining of GAPDH. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data. Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. As well as functioning as a glycolytic enzyme in cytoplasm, recent evidence suggests that mammalian GAPDH is also involved in a great number of intracellular |
| 保存條件 | Please store components at the temperatures indicated on the individual tube labels. The kit is stable for 6 months from the date of receipt. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產(chǎn)品介紹 |
Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. As well as functioning as a glycolytic enzyme in cytoplasm, recent evidence suggests that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of data appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age related neuronal diseases, such as Alzheimer's and Huntington's disease. GAPDH is expressed in all cells. It is constitutively expressed in almost all tissues at high levels. There are however some physiological factors such as hypoxia and diabetes that increase GAPDH expression in certain cell types. GAPDH molecule is composed of four 36kDa subunits. SWISS: P04406 Gene ID: 2597 |
| 產(chǎn)品圖片 |
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Immunohistochemical analysis of paraffin embedded mouse colon tissue slide using IHC0121 (GAPDH IHC Kit).
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Immunohistochemical analysis of paraffin embedded rat colon tissue slide using IHC0121 (GAPDH IHC Kit).
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Immunohistochemical analysis of paraffin embedded human colon tissue slide using IHC0121 (GAPDH IHC Kit).
Immunohistochemical analysis of paraffin embedded human lung carcinoma tissue slide using IHC0121 (GAPDH IHC Kit).
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
